Characterization of the gut microbiome in wild rocky mountainsnails (Oreohelix strigosa)

Jingchun Li,Bridget Chalifour

doi:10.1186/s42523-021-00111-6

Jingchun Li, Bridget Chalifour

Open Access

https://doi.org/10.1186/s42523-021-00111-6

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Abstract

BackgroundThe Rocky Mountainsnail (Oreohelix strigosa) is a terrestrial gastropod of ecological importance in the Rocky Mountains of western United States and Canada. Across the animal kingdom, including in gastropods, gut microbiomes have profound effects on the health of the host. Current knowledge regarding snail gut microbiomes, particularly throughout various life history stages, is limited. Understanding snail gut microbiome composition and dynamics can provide an initial step toward better conservation and management of this species.ResultsIn this study, we employed 16S rRNA gene amplicon sequencing to examine gut bacteria communities in wild-caught O. strigosa populations from the Front Range of Colorado. These included three treatment groups: (1) adult and (2) fetal snails, as well as (3) sub-populations of adult snails that were starved prior to ethanol fixation. Overall, O. strigosa harbors a high diversity of bacteria. We sequenced the V4 region of the 16S rRNA gene on an Illumina MiSeq and obtained 2,714,330 total reads. We identified a total of 7056 unique operational taxonomic units (OTUs) belonging to 36 phyla. The core gut microbiome of four unique OTUs accounts for roughly half of all sequencing reads returned and may aid the snails’ digestive processes. Significant differences in microbial composition, as well as richness, evenness, and Shannon Indices were found across the three treatment groups.ConclusionsComparisons of gut microbiomes in O. strigosa adult, fetal, and starved samples provide evidence that the host internal environments influence bacterial community compositions, and that bacteria may be transmitted vertically from parent to offspring. This work provides the first comprehensive report on the structure and membership of bacterial populations in the gastropod family Oreohelicidae and reveals similarities and differences across varying life history metrics. Strong differentiation between these life history metrics demonstrates the need for wider sampling for studies of dynamics of the snail gut microbiome.

Highlights

Complex microbial communities living in animal gastrointestinal tracts are known to play critical roles in a variety of biological processes [1]
This work provides the first comprehensive report on the structure and membership of bacterial populations in the gastropod family Oreohelicidae and reveals similarities and differences across varying life history metrics. Strong differentiation between these life history metrics demonstrates the need for wider sampling for studies of dynamics of the snail gut microbiome
Gut microbiomes may shift based on host developmental stages [12,13,14,15,16,17]

Summary

Introduction

Complex microbial communities living in animal gastrointestinal tracts are known to play critical roles in a variety of biological processes [1]. These communities can be comprised of a mix of horizontally acquired exogenous microbial taxa, and “core” taxa that confer selective advantages to the host and may be acquired vertically through the parent or horizontally [2]. Varying factors can influence animal gut microbiome compositions, including host diet,. Across the animal kingdom, including in gastropods, gut microbiomes have profound effects on the health of the host. Understanding snail gut microbiome composition and dynamics can provide an initial step toward better conservation and management of this species

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