Abstract
BackgroundSerial Analysis of Gene Expression (SAGE) is a new technique that allows a detailed and profound quantitative and qualitative knowledge of gene expression profile, without previous knowledge of sequence of analyzed genes. We carried out a modification of SAGE methodology (microSAGE), useful for the analysis of limited quantities of tissue samples, on normal human cervical tissue obtained from a donor without histopathological lesions. Cervical epithelium is constituted mainly by cervical keratinocytes which are the targets of human papilloma virus (HPV), where persistent HPV infection of cervical epithelium is associated with an increase risk for developing cervical carcinomas (CC).ResultsWe report here a transcriptome analysis of cervical tissue by SAGE, derived from 30,418 sequenced tags that provide a wealth of information about the gene products involved in normal cervical epithelium physiology, as well as genes not previously found in uterine cervix tissue involved in the process of epidermal differentiation.ConclusionThis first comprehensive and profound analysis of uterine cervix transcriptome, should be useful for the identification of genes involved in normal cervix uterine function, and candidate genes associated with cervical carcinoma.
Highlights
Serial Analysis of Gene Expression (SAGE) is a new technique that allows a detailed and profound quantitative and qualitative knowledge of gene expression profile, without previous knowledge of sequence of analyzed genes
There are reports of genes expressed by keratinocytes derived from a normal human epidermis and from mouse uterus carried out by Serial Analysis of Gene Expression (SAGE) [14,15,16,17]
The aim of our study was to describe the first compendium of expressed genes in normal cervical epithelium, which is composed mainly by keratinocytes strongly influenced by hormones
Summary
Serial Analysis of Gene Expression (SAGE) is a new technique that allows a detailed and profound quantitative and qualitative knowledge of gene expression profile, without previous knowledge of sequence of analyzed genes. A number of recent studies about gene expression profiles in in vitro HPV-infected cultured keratinocytes and from (CC) clinical samples have provided an initial notion of the changes in gene expression induced by HPV and in early CC [5,6,7,8,9,10]. The aim of our study was to describe the first compendium of expressed genes in normal cervical epithelium, which is composed mainly by keratinocytes strongly influenced by hormones. To achieve this we used SAGE, which is capable of producing an accurate molecular picture of cervical tissue based on expressed genes, as the main methodology. The present report describes a partial transcriptome of a sample derived from normal cervical epithelium used to construct a SAGE library with 30,418 sequenced tags
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