Abstract
The data presented here is related to the research article entitled “Characterization of the gila monster (Heloderma suspectum suspectum) venom proteome” by Sanggaard et al. in Journal of Proteomics [1]. The gila monster venom was collected, analyzed by 2D-gel electrophoresis and after Coomassie-Brilliant Blue staining the major spots were excised, subjected to in-gel trypsin digestion, and analyzed by LC–MS/MS. Subsequently, the venom proteins were identified based on de novo sequencing and homology searching. The mass spectrometry proteomics data have been deposited to the ProteomeXchange (dataset identifier PXD0001343), and in the present article we present an overview of the identified proteins. Protein identification failed for three of the selected spots, with the method described above. Instead, an iterative process, based on de novo sequencing, was employed.
Highlights
The data presented here is related to the research article entitled “Characterization of the gila monster (Heloderma suspectum suspectum) venom proteome” by Sanggaard et al in Journal of Proteomics [1]
PEAKS Studio 7.0 was employed for subsequent de novo sequencing and homology searching Jutland, Denmark (Danish breeder of gila monsters and beaded lizards) The data is accessible via this article, via the related research article [1], and at the ProteomeXchange consortium via the PRIDE partner repository with the dataset identifier PXD0001343 [2,3]
The obtained data were interrogated by PEAKS studio 7.0, which facilitates de novo sequencing and crossspecies homology searching [4,5], in order to identify the gila monster venom proteome (Fig. 1)
Summary
The aim of this project was to provide a global overview of the protein composition in venom from helodermatids, the archetypical venomous lizard species including beaded lizards and gila monsters (Fig. 1). Venom samples were collected from four species and the overall protein composition was assed by SDS-PAGE. A more detailed and targeted analysis of the venom proteome was performed focusing on the reticulated gila monster (Heloderma suspectum suspectum). The obtained data were interrogated by PEAKS studio 7.0, which facilitates de novo sequencing and crossspecies homology searching [4,5], in order to identify the gila monster venom proteome (Fig. 1). An iterative de novo sequencing-based method was developed for the analyses of LC–MS/MS data, which did not result in protein identification by the initial analyses by PEAKS
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