Abstract
SUMMARY The DNA of soybean chlorotic mottle virus (SoyCMV) has been isolated. When analysed by gel electrophoresis, the native DNA formed multiple bands resembling DNA from other caulimoviruses. The viral DNA was cloned in bacterial vectors. Cloned DNA was used to map restriction sites on the SoyCMV DNA. Comparison of cloned DNAs of SoyCMV and cauliflower mosaic virus (CaMV) by Southern transfer hybridization revealed the absence of significant sequence homology. Electrophoresis in denaturing gels showed that SoyCMV DNA had three single-strand discontinuities (‘gaps’), one in one strand and two in the other, and the positions of these gaps were mapped on the viral DNA. Potential primer sites for reverse transcription were detected in the cloned DNA by sequence analysis of regions around the gap sites. The results obtained definitely establish SoyCMV as a member of the caulimovirus group and suggest a replication mechanism for this virus similar to that described for CaMV.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
