Abstract
The complete genome sequence of the virulent bacteriophage PMBT3, isolated on the proteolytic Pseudomonas grimontii strain MBTL2-21, showed no significant similarity to other known phage genome sequences, making this phage the first reported to infect a strain of P. grimontii. Electron microscopy revealed PMBT3 to be a member of the family Siphoviridae, with notably long and flexible whiskers. The linear, double-stranded genome of 87,196 bp has a mol% G+C content of 60.4 and contains 116 predicted protein-encoding genes. A putative tellurite resistance (terB) gene, originally reported to occur in the genome of a bacterium, was detected in the genome of phage PMBT3.
Highlights
Pseudomonas grimontii was originally isolated from French natural mineral water in 2002 [1]
We present an analysis of the genome sequence of the novel virulent phage PMBT3, which was isolated from sewage at a municipal wastewater treatment plant located close to Kiel in Germany
The isolated phage PMBT3 produced small (0.5 mm in diameter), turbid plaques on P. grimontii strain MBTL2-21 lawns grown on Caso soft agar at room temperature
Summary
Pseudomonas grimontii was originally isolated from French natural mineral water in 2002 [1]. Only a few studies have investigated phages against strains of Pseudomonas species that cause food spoilage [14–16]. We present an analysis of the genome sequence of the novel virulent phage PMBT3, which was isolated from sewage at a municipal wastewater treatment plant located close to Kiel in Germany. The phage was detected in a double-layer agar assay using P. grimontii strain MBTL2-21 as the host bacterium. Phage PMBT3 showed lysis of Pseudomonas strains that are associated with milk spoilage. Phage PMBT3 could have the potential to make dairy foods safer and improve their shelf-life. To our knowledge, this is the first report of a bacteriophage infecting P. grimontii
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