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Abstract
Bloodstream infection with Candida fungal cells remains one of the most life-threatening complications among hospitalized patients around the world. Although most of the cases are still due to Candida albicans, the rising incidence of infections caused by other Candida strains that may not respond to traditional anti-fungal treatments merits the development of a method for species-specific isolation of Candida. To this end, here we present the characterization of the dielectrophoresis (DEP) response of Candida albicans, Candida tropicalis and Candida parapsilosis. We complement such characterization with a study of the Candida cells morphology. The Candida strains exhibited subtle differences in their morphology and dimensions. All the Candida strains exhibited positive DEP in the range 10–500 kHz, although the strength of the DEP response was different for each Candida strain at different frequencies. Only Candida tropicalis showed positive DEP at 750 kHz. The current results show potential for manipulation and enrichment of a specific Candida strain at specific DEP conditions towards aiding in the rapid identification of Candida strains to enable the effective and timely treatment of Candida infections.
Highlights
Candida species are one of the most prevalent fungal pathogens in hospitals around the world
Here we present the characterization of the dielectrophoresis (DEP) response of Candida albicans, Candida tropicalis and Candida parapsilosis
We present the morphological characteristics and a first study on the DEP response of three different Candida strains: Candida albicans, Candida parapsilosis, and Candida tropicalis; which are three of the most frequently isolated Candida strains from infected samples
Summary
Candida species are one of the most prevalent fungal pathogens in hospitals around the world. In the United States alone, 5%–10% of hospitalized patients will acquire a nosocomial infection and 80% of such infections are caused by Candida species [1]. While Candida albicans remains the most frequently isolated Candida strains from infected blood [4], the incidence of the infections caused by other species has increased significantly worldwide. A survey in European countries showed that around 50% infection was caused by Candida albicans, whereas incidence rates were 14% for each Candida glabrata and Candida parapsilosis, 7% for Candida tropicalis and 2% for Candida krusei [5]. In Chile, the most frequently isolated non-albicans species was Candida parapsilosis, followed by Candida tropicalis and Candida glabrata [6]. With an increased incidence of infections with different Candida species, there is a need for a method that allows for rapid identification of the Candida species, so that timely measures can be taken towards species-specific treatment of Candida infections
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