Characterization of the complete plastome of Ophrys aveyronensis, a Euro-Mediterranean orchid with an intriguing disjunct geographic distribution

Joris A M Bertrand,Anaïs Gibert,Christel Llauro,Olivier Panaud

doi:10.1080/23802359.2019.1670748

Abstract

Ophrys aveyronensis is an orchid with disjunct geographic distribution. For biogeographic and conservation purpose, we sequenced its complete plastome using Illumina data. The complete plastome is 146,816 bp in length, consisting of a pair of inverted repeats (IRs) of 25,006 bp, a large single-copy (LSC) region and a small single-copy region (SSC) of 80,495 and 16,309 bp, respectively. It was found to contain 133 genes, including 86 protein-coding genes, up to 39 trRNA genes and 8 rRNA genes. The overall GC content of the plastid genome is 36.9%. Phylogenetic inference confirms that O. aveyronensis is very close to O. sphegodes.

Highlights

Ophrys sphegodes Mill. subsp. aveyronensis (Wood 1983) or Ophrys aveyronensis (Delforge 1984) is an orchid species that was first described as endemic to a geographically restricted area in the ‘Grands Causses’ region (Southern France)
We used a CTAB 2X protocol to extract genomic DNA from an individual of Ophrys aveyronensis collected near Lapanousede-Cernon, France (N 43.98945 E 3.09045) with appropriate permit and deposited in the collection of the University of Perpignan Via Domitia under accession number 18-GS-049
The plastid genome of O. aveyronensis is a circular molecule of 146,816 bp in length, comprising a large single-copy (LSC) region and a small single-copy region (SSC) of 80,495 and 16,309 bp, respectively, separated by two inverted repeat regions (IR) of 25,006 bp (Figure 1)

Summary

Introduction

Ophrys sphegodes Mill. subsp. aveyronensis (Wood 1983) or Ophrys aveyronensis (Delforge 1984) is an orchid species that was first described as endemic to a geographically restricted area in the ‘Grands Causses’ region (Southern France). KEYWORDS Ophrys sphegodes aveyronensis; Bee orchid; Orchidaceae; chloroplast (cp) genome; illumina sequencing Aveyronensis (Wood 1983) or Ophrys aveyronensis (Delforge 1984) is an orchid species that was first described as endemic to a geographically restricted area in the ‘Grands Causses’ region (Southern France).

Results

Conclusion