Abstract
Sequences of the complete nuclear ribosomal DNA (rDNA) gene from five individual Paramphistomum cervi were determined for the first time. The five complete rDNA sequences, which included the 18S rDNA, the internal transcribed spacer 1 (ITS1), the 5.8S rDNA, the internal transcribed spacer 2 (ITS2), the 28S rDNA, and the intergenic spacer (IGS) regions, had a length range of 8,493–10,221 bp. The lengths of the investigated 18S, ITS1, 5.8S, ITS2, and 28S rDNA sequences, which were 1,994 bp, 1,293 bp, 157 bp, 286 bp, and 4,186 bp, respectively, did not vary. However, the IGS rDNA sequences had a length range of 577–2,305 bp. The 5.8S and ITS-2 rDNA sequences had 100% identity among the five investigated samples, while the identities among the IGS had a range of 53.7–99.8%. A comparative analysis revealed that different types and numbers of repeats were found within each ITS1 and IGS region, which may be related to the length polymorphism of IGS. The phylogenetic position of P. cervi in Paramphistomatidae was analyzed based on the 18S rDNA sequences. These results will aid in studying the intra- and interspecific variation of the Paramphistomatidae and the systematics and phylogenetics of Digenea.
Highlights
Paramphistomum cervi (Trematoda: Digenea: Paramphistomatidae), the representative species of the genus Paramphistomum, has adult flukes that customarily inhabit the rumen and immature worms that parasitize the gallbladder and reticulum of ruminants, including cattle, sheep, goat, and some wild mammals [1, 2]
All five complete ribosomal DNA (rDNA) sequences have been deposited in GenBank
There was no variation in the lengths of the 5.8S, internal transcribed spacer 2 (ITS2), and 28S rDNA regions obtained from five P. cervi samples in this study, which were 157 bp, 286 bp, and 4,186 bp, respectively
Summary
Paramphistomum cervi (Trematoda: Digenea: Paramphistomatidae), the representative species of the genus Paramphistomum, has adult flukes that customarily inhabit the rumen and immature worms that parasitize the gallbladder and reticulum of ruminants, including cattle, sheep, goat, and some wild mammals [1, 2]. There was no variation in the lengths of the 5.8S, ITS2, and 28S rDNA regions obtained from five P. cervi samples in this study, which were 157 bp, 286 bp, and 4,186 bp, respectively.
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