Characterization of the complete mitochondrial genome of the paddy frog Fejervarya multistriata (Anura: Dicroglossidae) and its phylogeny

Abstract

The mitochondrial genome has proven to be a highly successful resource for the investigation of evolution and population genetics. Here, we present the complete mitochondrial genome of Fejervarya multistriata. The circle genome was found to be 17,759 bp in length, containing 13 protein-coding genes, 23 transfer RNA genes, 2 ribosomal RNA genes, and 1 noncoding control region that are conserved in most Dicroglossidae mitogenomes. The total base composition of the F. multistriata mitogenome is 28.04% A, 29.82% T, 26.99% C, and 15.15% G, which is typical for Amphibious animals’ mitochondrial genomes. Eight tRNAs are encoded on the light strand (tRNA-Gln, tRNA-Ala, tRNA-Asn, tRNA-Cys, tRNA-Tyr, tRNA-Ser, tRNA-Glu and tRNA-Pro). Only one PCG is encoded on the light strand (ND6), whereas the other genes are located on the heavy strand. Phylogenetic analyses were performed on the concatenated dataset of 13 PCGs at nucleotide levels with maximum likelihood (ML) and Bayesian analysis (BI) methods. The results showed that F. multistriata and F. limnocharis integrated into a big branch and they have a close genetic relationship. This study could provide important molecular data for species identification and the phylogenetic relationship of Fejervarya and related species.