Abstract
Dracocephalum heterophyllum (Lamiaceae: tribe Mentheae) is an annual aromatic herb native to East Asia with a long record of human uses, including medicinal, alimentary, and ornamental values. However, no information is available about its molecular biology, and no genomic study has been performed on D. heterophyllum. Here, we report the complete chloroplast (cp) genome of D. heterophyllum and a series of comparative genomic analyses between this and closely related species of Lamiaceae. Results indicated that the cp genome has a typical circular structure of 150,869 bp in length, consisting of a long single-copy (LSC) region with 82,410 bp, a short single-copy (SSC) region with 17,098 bp, and two inverted repeat (IR) regions of 51,350 bp. A total of 133 genes were identified, including 37 tRNA genes, 8 rRNA genes and 88 protein-coding genes, with a GC content of 37.8%. The gene content, organization, and GC values observed here were similar to those of other Dracocephalum species. We detected 99 different simple sequence repeat loci, and the codon usage analysis revealed a preferential use of the Leu codon with an A/U ending. Comparative analysis of cp genome sequences revealed five highly variable regions with remarkably higher Pi values (>0.03). The mean Ka/Ks between D. heterophyllum and three other Dracocephalum species ranged from 0.01079 (psbB) to 1.0497 (ycf2). Two cp genes, ycf2 and rps11, were proven to have high ratios of Ka/Ks, implying that cp genes may had undergone positive selection in the evolutionary history. We performed multiple sequence alignments using the cp genome of 22 species and constructed maximum likelihood (ML) and Bayesian trees, and found that D. heterophyllum were more closely related to D. moldavica and D. palmatum. In addition, the phylogenetic relationships between Dracocephalum and other members of Lamiaceae were consistent with previous results. These results are valuable for further formulating effective strategies of conservation and management for species in Dracocephalum, as well as providing a foundation for future research on the genetic resources of Dracocephalum.
Highlights
The mint family, Lamiaceae, comprise more than 7000 species from about 236 genera and seven subfamilies distributed worldwide [1]
The assembled and annotated cp genome of D. heterophyllum was 150,869 bp in length and presented a typical tetra-partite circular structure with a pair of inverted repeats (IRs) of 51,350 bp each, which made the genome separate into two single-copy regions: a long single-copy (LSC) of 82,421 bp and short single-copy (SSC) of 17,098 bp (Figure 1)
Our results showed that the lengths of LSC, IR, and SSC regions were similar among the genomes of Lamiaceae
Summary
The mint family, Lamiaceae, comprise more than 7000 species from about 236 genera and seven subfamilies distributed worldwide [1]. Plants in this family include numerous herbs with medicinal and ornamental values, such as Agastache rugosa, Mentha canadensisis, Perilla frutescens, and Scutellaria baicalensis [2]. The genus Dracocephalum, commonly known as “Dragonheads”, comprises over 60 species of aromatic perennial herbs related to mints (tribe Mentheae) distributed in the northern temperate parts of the world. In China, 32 species and seven varieties of Dracocephalum are reported, mainly distributed in Northwest China [3]. Previous studies have reported that the main active component found in D. heterophyllum is effective calming the mind, conferring protection for hypoxic brain damage, is antibacterial, and can alleviate diseases such as high blood pressure, lymphadenitis, and cough [4,5]
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