Abstract
An aerobic, yellow-pigmented, bacteriochlorophyll a-producing strain, designated AAP5 (=DSM 111157=CCUG 74776), was isolated from the alpine lake Gossenköllesee located in the Tyrolean Alps, Austria. Here, we report its description and polyphasic characterization. Phylogenetic analysis of the 16S rRNA gene showed that strain AAP5 belongs to the bacterial genus Sphingomonas and has the highest pairwise 16S rRNA gene sequence similarity with Sphingomonas glacialis (98.3%), Sphingomonas psychrolutea (96.8%), and Sphingomonas melonis (96.5%). Its genomic DNA G + C content is 65.9%. Further, in silico DNA-DNA hybridization and calculation of the average nucleotide identity speaks for the close phylogenetic relationship of AAP5 and Sphingomonas glacialis. The high percentage (76.2%) of shared orthologous gene clusters between strain AAP5 and Sphingomonas paucimobilis NCTC 11030T, the type species of the genus, supports the classification of the two strains into the same genus. Strain AAP5 was found to contain C18:1ω7c (64.6%) as a predominant fatty acid (>10%) and the polar lipid profile contained phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine, sphingoglycolipid, six unidentified glycolipids, one unidentified phospholipid, and two unidentified lipids. The main respiratory quinone was ubiquinone-10. Strain AAP5 is a facultative photoheterotroph containing type-2 photosynthetic reaction centers and, in addition, contains a xathorhodopsin gene. No CO2-fixation pathways were found.
Highlights
We previously isolated a novel Sphingomonas sp. strain designated AAP5 from the alpine lake Gossenköllesee located in the Tyrolean Alps, Austria
The sampling was conducted in the clear alpine lake Gossenköllesee, Tyrolean Alps, Austria in September 2012
The plates were incubated aerobically at 25 ◦ C under 12-h-light/dark cycles until colonies were visible, which were screened for the presence of bacteriochlorophyll a (BChl a) using an infrared (IR) imaging system [26]
Summary
Licensee MDPI, Basel, Switzerland.Attribution (CC BY) license (https://creativecommons.org/licenses/by/ 4.0/).The genus Sphingomonas (Alphaproteobacteria) was originally proposed by Yabuuchi and coworkers [1] as a genus accommodating Gram-negative, strictly aerobic, nonsporulating, non-motile or motile, non-fermenting, chemoheterotrophic bacteria [2,3].Later, this genus was divided into four genera and genus Sphingomonas was redefined in sensu stricto [4]. Over time, several photoheterotrophic representatives of Sphingomonas were cultivated [5,6,7,8]. Sphingomonas are found in a wide range of environmental niches, such as soils [6,9,10], fresh and marine waters [11,12,13], plants [14,15], airborne dust [16,17], Microorganisms 2021, 9, 768. https://doi.org/10.3390/microorganisms9040768 https://www.mdpi.com/journal/microorganisms
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