Abstract
The 16S–23S intergenic spacer region from the ribosomal RNA (rRNA) operon of Bartonella bacilliformis was cloned and characterized. The spacer is 906 nucleotides (nt) in length and contains the genes encoding isoleucine-tRNA (tRNA Ile) and alanine-tRNA (tRNA Ala). The tRNA-encoding genes are separated by 122 nt and are centrally located in the 16S–23S spacer region, with approx. 300 flanking nt. Genes encoding tRNA Ile and tRNA Ala have 88.3 and 93.4% sequence identity, respectively, to the homologous genes of Rhodobacter sphaeroides.
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