Characterization of ‘Sun II’ oat monosomics through C-banding and identification of eight new ‘Sun II’ monosomics

Abstract

Monosomics are a powerful tool for genetic mapping in allopolyploid plant species such as oat (Avena sativa L., 2n=6x=42). A C-banded karyotype of the oat cultivar Sun II was compared with previously described oat karyotypes and was used to identify the missing chromosome in each line of Sun II aneuploids. These included new aneuploids, isolated among derivatives of oat haploids obtained from Sun II oat×maize crosses, along with the original Sun II aneuploid set which had been obtained by cytological screening of a Sun II population for spontaneous aneuploids. Eight new Sun II monosomics were identified among the derivatives of haploids from the oat×maize crosses, to give a total of 18 unique Sun II monosomic/nullisomic lines. All seven C-genome chromosomes are represented by Sun II monosomics. Chromosomes 13, 14 and 17 are not represented by Sun II aneuploids but are found in the Kanota monosomic series. Therefore, monosomics of some form are now available for all 21 oat chromosomes. A reciprocal translocation involving chromosomes 3C and 14, found in a portion of the original set of Sun II monosomic lines, was also described. No new translocations were detected in the Sun II×maize crosses.