Characterization of Streptococcus suis isolates recovered between 2008 and 2011 from diseased pigs in Québec, Canada

Abstract

In the present study we report the distribution of different serotypes of Streptococcus suis among strains isolated from diseased pigs in Québec, Canada, recovered between 2008 and 2011. Serotype 2 strains were further studied for the presence of the following virulence markers: suilysin (sly), muramidase-released protein (MRP), extracellular protein factor (epf) and the pilus encoded by the srtF cluster. Of 1004 field strains collected, 986 were confirmed to be S. suis by either the species-specific PCR targeting the gdh gene or by 16S rRNA gene sequencing analysis. Results showed that, although widely used, the species-specific PCR test can sometimes be misleading and fail to correctly identify some S. suis isolates. Serotypes 2, 3, 1/2, 4, 8 and 22 together represented 51% of S. suis strains (64.5% of typable strains). Results confirmed the relatively low prevalence of serotype 2 in North America, when compared to European and Asian countries. The vast majority of serotype 2 field strains (96%) belong to either the MRP+, srtF pilus+, epf−, sly− (52%) or the MRP−, srtF pilus−, epf−, sly− phenotypes (44%). Most non-typable strains (89%) presented high surface hydrophobicity, suggesting that these are poorly or non-encapsulated. Electron microscopy studies confirmed the lack of capsular polysaccharide in selected non-typable high hydrophobic strains. The role and pathogenesis of the infection caused by these strains remain to be elucidated.