Abstract
The spawn-inducing radial nerve factor (RNF) of the sea urchin, Strongylocentrotus purpuratus, is a heat-stable polypeptide. The molecular weight of this RNF, as estimated by sodium dodecyl sulfate polyacrylamide gel electrophoresis, is 5600 ± 800. The RNF induces sea urchin gonads to produce a nonprotein gonad factor. This gonad factor (GF), as well as 1-methyladenine (the starfish gonad factor), causes sea urchin testis fragments to spawn. GF co-chromatographs with 1-methyladenine on thin-layer chromatography plates.
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