Characterization of Soybean Genotypes on the Basis of Yield Attributing Traits and SSR Molecular Markers

Abstract

Soybean is well-thought-out to be a main crop as an important foundation of nutrients to humans and animals. The current investigation has been executed to recognize different soybean genotypes on account of diverse morpho-physiological traits and SSR molecular markers. Data for different morpho-physiological traits were documented from experiment conducted under field conditions in RBD design whereas molecular work was conducted in laboratory with 32 microsatellite markers to analyze the existence of possible diversity among different soybean genotypes. Morpho-physiological investigation evidenced the incidence of substantial magnitude of variability. Phylogenetic tree based on morpho-physiological traits grouped the genotypes into major and minor cluster. Major cluster had fifty genotypes while minor cluster had only three genotypes. Among polymorphic 32 microsatellite markers, the highest genetic diversity (0.66) was documented for the marker Satt520 whilst lowest (0.037) for the marker Satt557 with an average of 0.35. The highest PIC value also was 0.59 prearranged by same marker viz., Satt520 and lowest 0.036 by marker Satt557. An average major allele frequency was 0.69 while, an average PIC value was 0.32. Microsatellite markers-based data also congregated the genotypes into one major and one minor cluster. Molecular analysis based on microsatellite markers confirms the presence of genetic variability among genotypes under the investigation. Data obtained from the present research may contribute towards improvement of soybean genotypes to advance high yielding varieties by considering assorted genotypes with good agronomical traits in breeding scheme.