Characterization of some normal-phase liquid chromatographic stationary phases based on linear solvation energy relationships

Abstract

Characterization of a number of normal-phase liquid chromatography (NP-LC) stationary phases was attempted by estimating characteristic interaction constants for the stationary phases based on linear solvation energy relationships. Five characteristic interaction constants of the stationary phases, the lipophilicity ( v), polarizability ( r), dipolarity ( s), hydrogen bond (HB) acceptor basicity ( a) and HB donor acidity strength ( b) were determined by multiple regression analyses of logarithmic capacity factors ( k′) for a set of test solutes measured on them in the mobile phase of a given composition vs. the solute properties represented by characteristic molecular volume ( V x ), excess polarization ( R 2), dipolarity/polarizability ( π *), HB donor acidity ( α) and HB acceptor basicity ( β). Magnitudes of the five constants vary with the type of the stationary phases. The effect of polar modifier in the mobile phases on the interaction properties of the sorbents was also investigated.