Characterization of solid-supported dipalmitoylphosphatidylcholine membranes containing cholesterol.

Abstract

The incorporation of cholesterol into dipalmitoylphosphatidylcholine (DPPC) membranes, even in small amounts, has been shown to significantly alter the properties of the membrane. In this work, force-distance interaction profiles of DPPC membranes containing 8 mol % cholesterol obtained using the surface force apparatus are analyzed in the context of high-resolution structural characterization by atomic force microscopy and neutron reflectometry. The adhesion between the mixed membranes was greater than that for pure DPPC and was variable-depending on the number of defects in the outer membrane leaflets. These defects were only detectable by atomic force microscopy and had an average size of 230 ± 30 nm and 1-5% surface density in the outer leaflet. The adhesion between the membranes monotonically increased as the thickness of the membrane decreased-in direct correlation with the number of defects present (exposed hydrophobic groups) in the membrane contact region. Because of the low diffusion rate of gel-phase membranes, the interaction force profiles were stable and no membrane restructuring was observed.