Abstract
Nanobiotechnology is a promising area to cater human life. Biological methods for the synthesis of silver nanoparticles are relatively cost effective process. It was aimed at synthesizing silver nanoparticles using water and soil borne bacterial isolates, characterization of silver nanoparticles using UV-Vis spectroscopy and Fourier transform infra red (FTIR) spectroscopy and its effect on multi drug resistant bacterial isolate. The biosynthesis of silver nanoparticles was evaluated in both bacterial biomass and culture supernatant of Bacillus, Pseudomonas and Escherichia coli in the presence of 1mM of AgNO3.On the basis of physical appearances of silver nano particles, Pseudomonas sp. was selected for synthesis. The absorbance spectra of reaction mixture of bacterial biomass and supernatant show the strong peak at 420 nm, indicating the presence of silver nanoparticles (AgNPs) using UV-Vis and FTIR spectrophotometry. The influence of synthesized AgNPs was tested against multi drug resistant (MDR) Staphylococcus sp. on Mueller Hinton agar. The multi antibiotics resistant Staphylococcus sp. showed antibiotic sensitivity against the antibiotic discs impregnated with silver nanoparticles. The characteristics of silver nanoparticles revealed its possible use in biomedical field.
Highlights
IntroductionThe idea of nanotechnology was coinedby Professor Richard Feynman [1]
The present study emphasizes the use of bacteria for silver nanoparticles synthesis with potent biological effect such as characterization of silver nanoparticles having antibacterial activity
The silver nanoparticles were synthesized in both bacterial biomass and culture in the presence of 1mM of AgNO3
Summary
The idea of nanotechnology was coinedby Professor Richard Feynman [1]. Nanosilver has been used for more than 150 years in the form of colloidal silver, and registered as a biocidal agent in the United States [2]. Silver nitrate is often used as a precursor in the biosynthesis of silver nanoparticles. Silver nanoparticles (AgNPs) has being studied extensively in bionanotechnology mainly due totwomajor reasons, i) it isnon-toxicin nature, (ii) it is safe inorganic antibacterial agent and has ability of killing various kinds of disease causing microbes. Metallic nanoparticles exhibit several unusual optical, thermal, chemical and physical features [3]. Nanoparticles exist in several different morphologies such as spherical, cylindrical, platelets, tubes etc. Nanoparticles exist in several different morphologies such as spherical, cylindrical, platelets, tubes etc. [4]
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