Abstract
Purpose : To investigate the efficiency of silver nanoparticles synthesized by wet chemical method, and evaluate their antibacterial and anti-cancer activities. Methods : Wet chemical method was used to synthesize silver nanoparticles (AgNPs) from silver nitrate, trisodium citrate dehydrate (C 6 H 5 O 7 Na 3 .2H 2 O) and sodium borohydride (NaBH 4 ) as reducing agent. The AgNPs and the reaction process were characterized by UV–visible spectrometry, zetasizer, transmission electron microscopy (TEM) and scanning electron microscopy (SEM) equipped with energy dispersive spectroscopy (EDS). The antibacterial and cytotoxic effects of the synthesized nanoparticles were investigated by agar diffusion method and MTT assay respectively. Results : The silver nanoparticles formed were spherical in shape with mean size of 10.3 nm. The results showed good antibacterial properties, killing both Gram-positive and Gram-negative bacteria, and its aqueous suspension displayed cytotoxic activity against colon adenocarcinoma (HCT-116) cell line. Conclusion : The findings indicate that silver nanoparticles synthesized by wet chemical method demonstrate good cytotoxic activity in colon adenocarcinoma (HCT-116) cell lines and strong antibacterial activity against various strains of bacteria. Keywords : Wet chemical, Silver nanoparticles, Characterization, Diffusion method, Antibacterial, Cytotoxic activity
Highlights
Nanotechnology is probably one of the fastest developing sciences over the last few years
The cells were propagated in Dulbecco's modified Eagle's medium (DMEM) supplemented with 10 % heat-inactivated fetal bovine serum, 1 % L-glutamine, HEPES buffer and 50 μg/mL gentamycin
transmission electron microscopy (TEM) images recorded at high magnification morphology of the synthesized silver nanoparticles indicates that some of nanoparticles were spherical in shape with a smooth surface morphology while other were aggregated and irregular shaped
Summary
Nanotechnology is probably one of the fastest developing sciences over the last few years. The products of nanotechnology, nanoparticles, have a size < 100 nm and, due to their small size, have very high bioactivity [1]. Metal nanoparticles can be prepared and stabilized by physical and chemical methods [2]. Numerous approaches had been used to prepare silver nanoparticles (AgNPs). Examples of physical technique to prepare AgNPs are photochemical synthesis, laser ablation, phase transfer processes, microemulsion, microwave treatment [3], which had been used for preparation in both solution and solid phases of silver nanoparticles. Chemical methods are widely used to produce nanostructured materials due to their straight forward nature and their potential to produce large quantities of the final product. A variety of chemical approaches have been utilized to produce silver nanoparticles with different size distribution and different shapes [6]
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