Abstract
Metal nanoparticles are widely used in different areas such as biotechnology and biomedicine, for example in drug delivery, imaging and control of bacterial growth. The antimicrobial effect of silver has been identified as an alternative approach to the increasing bacterial resistance to antibiotics. Silver nanoparticles were synthesized by the green route using the Geranium extract as a reducing agent. The characterization was carried out by the techniques of UV-Vis spectrophotometry, transmission electron microscopy (TEM), high-resolution transmission electron microscopy (HRTEM), X-ray emitted photoelectron spectroscopy (XPS) and X-ray diffraction. Nanoparticle diameters between 15 and 50 nm were obtained and the interplanar spaces calculated from the electron diffraction pattern corresponding to a mixture of silver with 4H and FCC structures. To determine the minimum inhibitory concentration of silver nanoparticles (AgNPs) on the Pseudomonas aeruginosa bacteria (ATCC-27853), different concentrations of colloidal solution 0.36, 0.18, 0.09 and 0.05 μg/mL were evaluated as a function of the incubation time, measuring the inhibition halo and colony forming unit (CFU) during 0, 2 and 4 h of incubation. The minimum inhibitory AgNPs concentration (MIC) is 0.36 μg/mL at 0 h while the concentration of 0.18 μg/mL presents a total inhibition of the bacterium after 2 h. For the rest of the dilutions, gradual inhibitions as a function of time were observed. We evaluate the antibacterial effect of silver nanoparticles obtained by a green methodology in Pseudomonas aeruginosa bacteria. Finally, the colloidal nanoparticle solution can be an antibacterial alternative for different biomedical approaches.
Highlights
IntroductionIn the field of materials science, nanoparticles are widely used for different applications, because their morphological properties, size, structure and distribution can be controlled and directed in specific applications [5,6,7,8,9]
Nanotechnology has revolutionized several areas of science in the search for new alternatives to improve the living conditions of humans, both in health and environmental fields [1,2,3,4].Crystals 2020, 10, 395; doi:10.3390/cryst10050395 www.mdpi.com/journal/crystalsIn the field of materials science, nanoparticles are widely used for different applications, because their morphological properties, size, structure and distribution can be controlled and directed in specific applications [5,6,7,8,9]
The obtained AgNPs have a diameter up to 50 nm. We demonstrated their antibacterial activity against Pseudomonas aeruginosa (ATCC-27853) using different concentrations of the colloidal solution as a function of incubation time
Summary
In the field of materials science, nanoparticles are widely used for different applications, because their morphological properties, size, structure and distribution can be controlled and directed in specific applications [5,6,7,8,9]. These methodologies are a bit expensive and, it is complicated to have this type of equipment in conventional laboratories. The most studied nanoparticles are silver, due to their lack of toxicity and better antibacterial properties
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