Characterization of Shrimp Oil from Pandalus borealis by High Performance Liquid Chromatography and High Resolution Mass Spectrometry.

Guangling Jiao,Ian Burton,Stephen Ewart,Marie-Hélène Thibault,Nadia Tchoukanova,Claude Pelletier,Joseph Hui,Yahia Djaoued,Kathryn Ewart,Balaji Subramanian,Junzeng Zhang,Jacques Gagnon,Josée Boudreau

doi:10.3390/md13063849

Guangling Jiao, Ian Burton + Show 11 more

Open Access

https://doi.org/10.3390/md13063849

Copy DOI

Abstract

Northern shrimp (Pandalus borealis) oil, which is rich in omega-3 fatty acids, was recovered from the cooking water of shrimp processing facilities. The oil contains significant amounts of omega-3 fatty acids in triglyceride form, along with substantial long-chain monounsaturated fatty acids (MUFAs). It also features natural isomeric forms of astaxanthin, a nutritional carotenoid, which gives the oil a brilliant red color. As part of our efforts in developing value added products from waste streams of the seafood processing industry, we present in this paper a comprehensive characterization of the triacylglycerols (TAGs) and astaxanthin esters that predominate in the shrimp oil by using HPLC-HRMS and MS/MS, as well as 13C-NMR. This approach, in combination with FAME analysis, offers direct characterization of fatty acid molecules in their intact forms, including the distribution of regioisomers in TAGs. The information is important for the standardization and quality control, as well as for differentiation of composition features of shrimp oil, which could be sold as an ingredient in health supplements and functional foods.

Highlights

Northern shrimp (Pandalus borealis), the most abundant cold-water shrimp in the North Atlantic and Pacific areas, have been widely fished since the early 1900s
We investigated the lipid classes, total profile of FAs and intact structural information of TAGs and astaxanthin esters of Northern shrimp oil derived from processing byproducts
Analysis (Figure S1) suggested that the chromatographic procedure had successfully removed all triacylglycerols from shrimp oil and provided astaxanthin-rich fraction for further analysis

Summary

Introduction

Northern shrimp (Pandalus borealis), the most abundant cold-water shrimp in the North Atlantic and Pacific areas, have been widely fished since the early 1900s. The protein content was found to vary between 33% and 40% of the dry weight, while the chitin content varied between 17% and 20%. The shrimp shells had low lipid content, varying from 0.3% to 0.5% of the dry weight. This material is a rich source of nutrients such as chitin polymers [4,5], carotenoid pigments (mainly astaxanthin) [6,7] and polyunsaturated fatty acids (PUFAs) [8] that are valuable ingredients in the aquaculture industry

Methods

Results

Discussion

Conclusion