Characterization of sheep liver and lung microsomal ethylmorphine N-demethylase

Abstract

1. 1. Ethylmorphine N-demethylase activity of the sheep liver and lung microsomes was reconstituted in the presence of solubilized microsomal cytochrome P-450, NADPH-cytochrome c reductase and synthetic lipid, phosphatidylcholine dilauroyl. 2. 2. The K m of the lung microsomal ethylmorphine N-demethylase was calculated to be 4.84 mM ethylmorphine from its Lineweaver-Burk graph and lung enzyme was inhibited by its substrate, ethylmorphine, when its concn was 25 mM and above, reaching to 67% inhibition at 50 mM concn. 3. 3. The Lineweaver-Burk and Eadie-Hofstee plots of the liver enzyme were found to be curvilinear. From these graphs, two different K m values were calculated for the liver enzyme as 4.17 mM and 0.40 mM ethylmorphine. 4. 4. Ethylmorphine N-demethylase activities of both liver and lung microsomes were inhibited by NiCl 2, CdCl 2 and ZnSO 4. Ethylalcohol inhibited N-demethylation of ethylmorphine in lung and liver microsomes. 5. 5. Acetone (5%) slightly enhanced the N-demethylase activity of the liver enzyme, whereas 5% acetone completely inhibited the lung enzyme. 6. 6. Phenylmethylsulfonyl fluoride at 0.10 mM and 0.25 mM concn had no effect on liver enzyme activity, while at these concns, it inhibited the activity of the lung enzyme by about 35%.