Abstract
Fifty nonpathogenic and insect-pathogenic strains of Serratia entomophila were investigated with respect to their genomic macrorestriction patterns and plasmid profiles. Genomic DNA fingerprints obtained after XbaI restriction and rotating field electrophoresis (RFE) were specific for almost each strain and differed significantly from other Serratia spp. No association was found between DNA fragment pattern and pathogenicity, but isolates from the same biotype and of common geographical origin shared a high degree of similarity (common bands). Results obtained indicate the genomic DNA macrorestriction analysis can be a valuable method for monitoring the fate of S. entomophila strains released into the environment. Plasmid profiles are indicative for the pathogenicity status of the host and show strain-specificity in some cases.
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