Abstract
Sediment microbial communities play an important role in lake trophic status. This study determined millions of Illumina reads (16S rRNA gene amplicons) to compare the bacterial communities in moderately eutrophic, lightly eutrophic, and moderately trophic regions using a technically consistent approach. The results indicated that the sediments from moderately eutrophic and trophic lake had the higher bacterial diversity than lightly eutrophic lake. Proteobacteria was the most abundant phylum (22.7%–86.2%) across samples from three regions. The sediments from moderately eutrophic region were enriched with Chloroflexi and Nitrospirae. Alphaproteobacteria, Gammaproteobacteria, and Bacteroidetes were enriched in the sediments from lightly eutrophic lake. The sediments from moderately trophic lake contained a high abundance of Acidobacteria and Deltaproteobacteria because of the low pH of the sediments in this lake. In moderately eutrophic region, Nitrospira held an absolute predominance, while Lysobacter and Flavobacterium were the most predominant genera in lightly eutrophic region. Temperature was the main factor influencing the bacterial community in the three lakes. The bacterial communities in the sediment samples obtained from moderately eutrophic lake were associated with nutrient concentration, whereas organic matter and total nitrogen contents mainly influenced the bacterial communities in sediments obtained from lightly eutrophic lake and moderately trophic lake, respectively.
Highlights
Lake sediment is one of the main media for nutrient transformation and migration and can act as either nutrient source or pool to the overlying water column; sediments influence the nutrient contents of lakes
This study investigated the bacterial communities in sediments obtained from the three lakes with different trophic statuses using Illumina Miseq high-throughput sequencing technology
The diversity of Chloroflexi and Nitrospirae was more abundant in the sediment samples obtained from Gonghu Bay of Taihu Lake and Huangda Lake than in the samples obtained from East Dongting Lake, and significant differences (p < .01) were observed among the samples obtained from these lakes
Summary
Lake sediment is one of the main media for nutrient transformation and migration and can act as either nutrient source or pool to the overlying water column; sediments influence the nutrient contents of lakes. Biogeochemical cycles, especially in sediments, are greatly influenced by microbial communities (Fang et al, 2015; Liu & Liu, 2013; Song, Li, Du, Wang, & Ding, 2012), and changes in. Shallow fresh water lakes with different trophic statuses are commonly found in plains. Trophic status and bacterial community are closely related, and understanding the differences in bacterial community structure of sediments in lake with different trophic statuses is crucial. In Eastern Plain of China, a large number of shallow fresh water lakes with different trophic statuses exist, and the sediments in these lakes greatly influence the trophic status of these lakes (Berglund, Larsson, Ewald, & Okla, 2001; Trolle, Hamilton, & Pilditch, 2010). DNA was diluted to 10 ngμl−1 using sterile ultrapure water and stored at −80°C for further analysis
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