Characterization of Salmonella spp. and E. coli Strains Isolated from Wild Carnivores in Janos Biosphere Reserve, Mexico.

Abstract

Simple SummaryEmerging diseases (EIDs) represent a constant challenge in public health. With the recent emergence of new pathogens, some questions about the mechanisms and sites where they are generated have aroused interest. Natural environments could be the sites where pathogenic microorganisms find the conditions to generate new variants. It has been established that approximately 60.3% of EIDs are caused by potentially zoonotic pathogens, of which more than half are thought to have originated from wild individuals. In this aspect, carnivores can play an important role in the dynamics of various diseases, since there are species that are widely distributed, roam large areas, and can be carriers of a wide range of microorganisms, some of which are zoonotic. The results obtained in this work show that different species of wild carnivores can be carriers of atypical strains of pathogenic microorganisms, which shows that natural environments can represent important sites for the study of EIDs.Enterobacteriaceae are considered one the most important zoonotic pathogens. In this study, we analyzed the characteristics of E. coli and Salmonella spp. strains present in carnivores from Janos Biosphere Reserve, Mexico. These microorganisms had been isolated from a wide range of domestic and free-range animals, including wild carnivores. Fifty-five individuals were sampled, and the presence of Salmonella and E. coli was determined by bacteriological standard methods. Strains isolated were characterized by molecular methods and in vitro infection assays. Eight different species of carnivores were captured, including coyotes (Canis latrans), gray fox (Urocyon cinereoargenteus), desert foxes (Vulpes macrotis), striped skunks (Mephitis mephitis), hooded skunks (Mephitis macroura), lynxes (Lynx rufus), raccoons (Procyon lotor), and badgers (Taxidea taxus). Salmonella spp. and E. coli were isolated from four species of carnivores. Five Salmonella spp. strains were isolated, and their molecular characterization revealed in three of them the presence of fimbrial and virulence genes associated with cell invasion. In vitro evaluation of these strains showed their capability to invade human Hep2 cells. Sixty-one E. coli strains were isolated; different serotypes and phylogroups were observed from these strains. Additionally, the presence of virulence genes showed differently.