Characterization of |rpsL and rrs genes of aminoglycoside-resistant clinical isolates of Mycobacterium tuberculosis in Wuhan

Abstract

Objective To characterize the rpsL and rrs genes of aminoglycoside-resistant clinical isolates of Mycobacterium tuberculosis in Wuhan.Methods Sixty-nine clinical isolates of Mycobacterium tuberculosis were collected from Wuhan Medical Treatment Center,including 35 streptomycin(SM)-resistance isolates(4 were also resistant to kanamycin) and 34 isolates susceptible to SM(20 were pan-susceptible).Mutation characteristics of rpsL and rrs genes were analyzed with PCR direct sequencing.Results Of the 35 SM-resistant clinical isolates,26(74.3%) carried mutations in rpsL gene,with rpsL Lys43Arg and Lys88Arg being the most common rpsL mutations,and one new mutation in codon 88 of rpsL(Lys88Glu) was detected.Four types of mutations in rrs gene in 13 resistant isolates(37.1%) including A1401G(17.1%,6/35),A514C(14.2%,5/35),G1487A(2.9%,1/35) and C517T(2.9%,1/35) were revealed,and one pan-susceptible isolate presenting C1029T mutation in rrs gene was detected.The mutations in rrs gene were only found in 3 kanamycin-resistant isolates,and G1487A mutation was detected in rrs gene,which was first reported.Conclusion Aminoglycoside-resistance of Mycobacterium tuberculosis is related to rpsL and rrs gene mutations,and the characteristics of these genes are diverse due to geographical differences.