Abstract
Riboflavin‐Binding Protein (RBP) is a glycophosphoprotein responsible for the transport of the vitamin Riboflavin in oviparous and other species. Evidence of copper binding by RBP suggests that the protein plays a role in copper transport. Identification and characterization of the specific RBP:Copper binding site is hindered by the lack of assay methods for the native or recombinant proteins. An improved protocol to purify and identify RBP was developed using Western blot methods adapted and optimized using rabbit antibody for identification. These methods can be used for purification and identification of recombinant RBP proteins.
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