Characterization of respiratory-related neurons in the isolated brainstem of the frog

Abstract

Using intra- and extracellular recording techniques we examined the spontaneous discharge and membrane properties of respiratory-related neurons in isolated brainstem preparations of the frogs Rana catesbeiana and Rana pipiens that display spontaneous respiratory related activity in vitro. We observed neurons that depolarize during the fictive lung ventilation cycle as well as neurons that depolarize during the non-lung ventilation phase. Respiratory-related neurons demonstrated significant decreases in membrane input resistance during the fictive lung ventilation cycle but showed no evidence of voltage-dependent membrane conductances activated near resting membrane potential. Furthermore, respiratory neurons showed little spike frequency adaptation, their oscillatory activity was not dissociated from the global respiratory motor output following imposed changes in membrane potential, and spontaneous fluctuations in membrane potential were not observed following reversible interruption of respiratory burst activity by application of solutions low in calcium and high in magnesium. Taken together these results suggest that bulbar respiratory neurons in the isolated frog brainstem sampled in our study do not display endogenous bursting characteristics. Rather, they are strongly influenced by synaptic input.