Abstract
AbstractPolymerase chain reaction primers for a total of 132 nuclear‐encoded microsatellites originally developed from genomic libraries for red drum Sciaenops ocellatus produced reliable and consistent amplifications in the closely related spotted seatrout Cynoscion nebulosus. Thirty‐three of the primers amplified microsatellites that were monomorphic among a sample of 30 individuals, while 12 of the remaining 99 polymorphic loci exhibited possible null alleles. This provides a set of 87 microsatellite loci that will be highly useful in future genetic studies related to the stock enhancement and culture of spotted seatrout. Spotted seatrout comprise an important recreational fishery in the bays and estuaries in U.S. waters of the Gulf of Mexico and western Atlantic Ocean, and stock enhancement via supplementation with hatchery‐reared individuals is ongoing, planned, or under consideration in several southern states.
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