Abstract
In the rat lacrimal gland, cholinergic agonists induce a large protein secretion through muscarinic receptors. The activation of these receptors stimulates phospholipase C that hydrolyses phosphatidylinositol 4,5-bisphosphate producing inositol 1,4,5-trisphosphate (IP3) and diacylglycerol (DAG). The roles of IP3 and DAG as second messengers are now well documented. IP3 releases calcium from a non mitochondrial intracellular store which increases the cytosolic free calcium concentration. The released calcium may stimulate secretion directly, or may act with calmodulin to activate specific kinases that phosphorylate specific proteins to induce secretion. DAG activates protein kinase C (PKC), a calcium and phospholipid-dependent protein kinase, which also may phosphorylates specific proteins to evoke secretion.KeywordsPhorbol EsterLacrimal GlandKrebs Ringer Bicarbonate BufferCytosolic Free Calcium ConcentrationDE52 ColumnThese keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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