Characterization of Pythium Species Associated With Greenhouse Floriculture Crops in Michigan.

Abstract

Michigan ranks third in the United States for the wholesale value of floriculture products, with an estimated value of $375.7 million. Seedling damping-off and root and crown rot are commonly caused by Pythium spp. and are important problems for greenhouse growers. Pythium spp. associated with Michigan's floriculture crops were characterized as a means to improve current management strategies. During 2011 and 2012, potted poinsettias with root rot symptoms were sampled from nine greenhouses located in Kent, Kalamazoo, and Wayne counties. In 2013, from the same three counties, symptomatic geranium and snapdragon bedding plants were sampled from 12 greenhouses. Additionally, symptomatic hibiscus and lantana plants were sampled at one greenhouse facility. Isolates were confirmed to be Pythium spp. via morphology and sequencing of the ITS region. A total of 287 Pythium spp. isolates were obtained from poinsettias and 726 isolates from geranium, snapdragon, hibiscus, and lantana. Seven Pythium spp., and a group of isolates determined as Pythium sp. 1 were identified. The most prevalent species were P. irregulare, P. ultimum, and P. aphanidermatum. A subset of isolates was chosen for pathogenicity and mefenoxam sensitivity testing. Six of the species were virulent to germinating geranium seeds. Most P. ultimum and P. cylindrosporum isolates tested were intermediate to highly resistant to mefenoxam, whereas most P. aphanidermatum isolates were sensitive. This study suggests that Pythium spp. recovered from Michigan greenhouses may vary depending on the host, and that mefenoxam may not be effective to control P. ultimum or P. cylindrosporum.