Abstract
The plant diseases caused by the Pseudomonas syringae сomplex bacteria are economically important and occur worldwide on various plants, and it is as a pathogen that has not been the object of studies and little is known about its epidemiology in Kyrgyzstan. The conventional phenotypic (LOPAT, API tests) and PCR-assisted isolation were used for the identificationof Pseudomonas syringae pv. syringaе isolates from the affected organs of local stone fruits, such as peach (Prunus persica), cherry (Prunus subgen), apricot (Prunus armeniaca), and plum (Prunus salicina) samples taken from the Chy, Issuk-Kul, and Batken regions of the country. 16S rRNA gene amplification was performed with primers 27F (5'-AGA GTT TGA TCC TGG CTC AG -3') and 907R (5 '–CCG TCA ATT CCT TTG AGT TT-3') for the identification of obtained P.syringae pv. syringaе isolates. From 40 primary isolates of Gram-negative rod-shaped bacteria, 12 were identified as Pseudomonas syringae pv. syringae, while the remaining isolates were identified as bacteria from Stenotrophomonas, Xanthomonas, Erwinia genera. The antagonist bio control agent—Streptomyces bacteria strains were screened and selected against the bacterial canker pathogen in in vitro experiments and on apricot seedlings in vivo conditions. Obtained results could encourage to develop a local bio-product based on this bioagent for spraying stone fruits with the initial manifestation of disease symptoms and to conduct preventive treatments in the fall and spring to increase the plant's resistance to pathogens.
Highlights
The plant diseases caused by the Pseudomonas syringae сomplex bacteria are economically important and occur worldwide on various plants, and it is as a pathogen that has not been the object of studies and little is known about its epidemiology in Kyrgyzstan
The conventional phenotypic (LOPAT, API tests) and polymerase chain reaction (PCR)-assisted isolation were used for the identification of Pseudomonas syringae pv. syringaе isolates from the affected organs of local stone fruits, such as peach (Prunus persica), cherry (Prunus subgen), apricot (Prunus armeniaca), and plum (Prunus salicina) samples taken from the Chy, Issuk-Kul, and Batken regions of the country. 16S rRNA gene amplification was performed with primers 27F (5'-AGA GTT TGA TCC TGG CTC AG -3') and 907R (5 '–CCG TCA ATT CCT TTG AGT TT-3') for the identification of obtained P.syringae pv. syringaе isolates
In observed sites P. syringae, pv. syringae isolates were obtained from infected organs of pome trees like Peach (Prunus persica), Cherry (Prunus subgen), Plum (Prunus salicina)
Summary
The plant diseases caused by the Pseudomonas syringae сomplex bacteria are economically important and occur worldwide on various plants. Syringae can cause diseases in more than 180 plant species including annual and perennial plants, fruit trees, ornamentals, and vegetables (Little et al, 1998; Agrios, 2005; Lamichhane et al, 2014; Lamichhane et al, 2015). P. syringae bacteria as the ubiquitous pathogen of numerous plant species has a special name—P. syringae sensu lato—proposed by (Morris et al, 2013). To protect plants from diseases caused by P. syringae pathogen, such bio-control bacteria like P. aeruginosa strain D4, Bacillus stratosphericus strain FW3, Paenibacillus polymyxa AC -1 were screened in vitro and in vivo trials. The biocontrol efficiency of the potential strains on the S. lycopersicum plant against P. syringae pathogen was resulting in growth promotion and in vivo antagonistic activity (Durairaj et al, 2018). The biocontrol ability of a wild-type B. subtilis strain 6051 against the bacterial pathogen P. syringae pv
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