Abstract
Publisher Summary Human cerebrospinal fluid (CSF) is an ideal source for identifying biomarkers for neurodegenerative diseases such as Alzheimer's disease (AD), Parkinson's disease (PD), and dementia with Lewy bodies (DLB). Proteomics has been used to analyze CSF in order to discover disease-associated proteins and elucidate the basic molecular mechanisms that either cause, or result from, central nervous system disorders. To identify as many CSF proteins in well-characterized healthy young subjects as possible, sodium dodecyl sulfate- polyacrylamide gel electrophoresis (SDS-PAGE) was used to prefractionate the CSF proteins before further separation by multidimensional liquid chromatography and analyzed with LCQ or LTQ-FT mass spectrometry (MS). LCQ-MS/ MS identified 466 proteins and LTQ-FTMS/MS identified 608 proteins, which was 30% over those identified by LCQ-MS/MS. Issues related to sample preparation, proteomic instrumentation, and database search are discussed further in the context of characterization of human CSF proteome.
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