Characterization of Protein Capacity of Nanocation Exchanger Particles as Filling Material for Functional Magnetic Beads for Bioseparation Purposes

Abstract

In this study we describe the synthesis and characterization of nanocation exchanger particles (NCEX) as the functional filling material for magnetic beads. Polystyrene NCEX particles were synthesized from styrene via a mini-emulsion polymerization. The coupling of cation exchanger groups was done with chlorosulfuric acid after the polymerization reaction. The NCEX particles have an average diameter of 160-260 nm. Their ion exchange capacity amounts up to 4.58 mval/g. In an adsorption experiment it was possible to adsorb 192 mg lysozyme/g NCEX. Depending on the equilibrium concentration of lysozyme in the bulk solution 70-85% of the attached protein was desorbed. NCEX particles were used to produce magnetic beads with cation exchanger properties. Therefore an innovative production process for the synthesis of magnetic beads from different single components was used. The produced magnetic beads contained 40 wt % NCEX material and showed an ion exchanger capacity of 2 mequiv/g. It was possible to adsorb 75 mg lysozyme/g magnetic beads with a maximum recovery rate of 95%.