Abstract
Cell cultures of prostatic epithelial tissue provide a model allowing the study of a single factor on prostatic epithelium without the complicating influences of the stroma. Investigators have developed methods of establishing prostatic epithelium in monolayer culture that maintain good differentiated structure and function, however, even these systems do not maintain the histological organization or columnar polarity of the epithelial cells and thus leave something to be desired. Work by Moscona and Douglas et al., have shown that epithelial cells cultured on a 3-dimensional collagen sponge matrix (GELFOAM, UPJOHN CO.) are capable of reaggregating to form histological structures similar to those found in the intact organ (e.g. alveolar-like structures in the lung, thus they are called organotypic cultures). Engle jat ell., have shown that this superior morphology is also reflected in an increased response of these cells to hormones ini vitro and thus reflects an increase in the retention of differentiated function.
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