Abstract
Lipases constitute as top three most important group of enzymes along with carbohydrases and proteases, and are widely used in various industries. In particular, lipase that perform high activity at low temperatures, or referred as cold adapted lipase (CLPs) considered as attractive catalyst due to its activity at low temperature. This unique feature is the main advantage of cold adapted lipase utilization because it requires a low energy source that is correlated with lower production costs and energy. In addition, reactions occur in cold temperatures may result in better product quality. The purpose of this research was to perform screening and characterization of bacterial cold adapted lipase from seafood cold storage. Among 53 isolates, Kr_16_30, TI_37_14 and Kr_16_28 showed the highest activity with 4.12 U/mL; 3.87 U/mL and 3.21 U/mL, respectively. Isolates Kr_16_30 seemed to be typical cold adapted lipase with optimum temperature at 20°C and pH 7. Isolates Kr_16_28 performed highest lipolytic activity at 30°C while TI_37_14 suspected to be similar to typical mesophilic lipase with optimum temperature at 40°C. Species identification based on 16s rRDA sequencing revealed that isolates Kr_16 30 and Kr_16 28 are belong to genus Pseudomonas and Bacillus, repectively.
Highlights
In recent decades, enzymes have received great attention in the effort to reduce chemical-based reactions in industrial process
Lipolytic activity is characterized by the appearance of white precipitation around the bacterial colonies
Various studies focused on the exploration and use of lipases that are active at extreme temperatures, due to industrial processes that are mostly carried out at high temperatures
Summary
Enzymes have received great attention in the effort to reduce chemical-based reactions in industrial process. It produces less waste, require less energy, reduce the risk of pollution and potentially improve product quality Kavitha (2016). Lipase (acyl glycerol hydrolases, EC 3.1.1.3) is one of most important enzymes with a wide range of applications in biotechnology, food, chemical, pharmaceutical, biodiesel and other industrial fields. Temperature seemed to be one of the most crucial environmental factors in enzymatic reactions. Higher temperatures, which is often applied to industrial processes, will increase the rate of reaction, and accelerate the deactivation of enzymes. Reactions at high temperatures will increase production costs Cowan (2010)
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