Abstract
Characterization of Potato Virus M epitopes with the use of synthetic peptides
Highlights
The antigenic analysis of two Potato Virus M Ukrainian strains (PVM Ul and U7) was carried out with the use of three monoclonal antibodies (MAbs M6D5, M9G1 and M4C1) [1]
The fractions collected were tested by indirect ELISA using three MAbs, M6D5, M4C1 and M9G1
Cognise any thermolysinic fraction while MAbs M6D5 and M9G1 reacted with fraction 17
Summary
The antigenic analysis of two Potato Virus M Ukrainian strains (PVM Ul and U7) was carried out with the use of three monoclonal antibodies (MAbs M6D5, M9G1 and M4C1) [1]. The synthetic peptide P14, corresponding to the tryptic fragment 2 Glu-Lys35, inhibited the MAbs M6D5 and M9G1 interaction with In previous work [1 ] we pointed out that lack of reaction between MAb M9G1 and lengthened peptides 22Glu/Gly-Arg44 may be caused by structural changes in the C-terminal region of this fragment after protein cleavage by trypsin.
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