Characterization of potassium solubilizing bacteria isolated from corn rhizoplane

Abstract

Potassium is one of the most important elements in plant growth and development. Most of the potassium reserves on Earth are in insoluble mineral form, which represents a limitation for the absorption of this nutrient by plants. Some microorganisms can solubilize the mineral forms of potassium. This study aimed to isolate and identify potassium solubilizing bacteria resident in corn rhizoplane. To do this, bacteria that formed a solubilization halo around the colony on solid Aleksandrov culture medium were selected. These bacteria were then characterized considering the appearance of the colonies and cell morphology and were identified by partial 16S rDNA sequencing. Their solubilizing and potassium-releasing capacity was determined under different conditions of temperature, pH, and salinity, using potassium feldspar and muscovite as insoluble sources of potassium. Eight strains identified within the genera Paenibacillus, Lysinibacillus, Arthrobacter, Bacillus, Pseudomonas, and Stenotrophomonas were obtained. The release of potassium from feldspar was favored at 28 and 30°C, pH 7.5 and a saline concentration of 4 g L-1, while in the presence of muscovite the best conditions were 30 and 37°C, pH 5.5 and 7.5, and 4 g L-1 of NaCl. The most efficient strains were Bacillus sp. INCA-FRc7 and Bacillus sp. INCA-FRc19x with yields of up to 2.095 mg L-1. These strains could become alternatives to the use of potassium fertilizers and contribute to the ecological sanitation of the agroecosystems.