Abstract
The fast and reliable analysis of carbohydrate mixtures from a synthetic reaction or isolated from a biological sample is a daily task in many laboratories. These components are highly polar and in most of the cases they are difficult to detect. The porous graphitic carbon (PGC) column was suggested in several papers as a suitable tool for carbohydrate analysis. In this pilot study the carbohydrate substrates and reaction products of amylases were analyzed. The characterization of the column was performed with the enzyme research standards and we expected to develop a method using PGC column for later examinations. It was found that the PGC column has some reversed phase characteristics and also some normal phase characteristics. Also the electron densities of the components play an important role in the observed retention.
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