Characterization of polysaccharides from Gynura procumbens with relation to their antioxidant and anti-glycation potentials

Abstract

Abstract Three polysaccharide fractions, namely CPGP-2-I, CPGP-2-II and CPGP-2-III were purified by DEAE-52 cellulose and Sephadex G-100 chromatographic columns from Gynura procumbens leaves, and their structural characteristics, antioxidant and anti-glycation capacities were investigated. The CPGP-2-I, CPGP-2-II and CPGP-2-III mainly composed mannose, galactose and glucose and had molecular weight of 10.4, 5.9 and 3.2 kDa, respectively. Antioxidant and anti-glycation abilities showed significant in concentration-dependent manner within range 10–200 μg/mL. The ability of CPGP-2-I to scavenging DPPH•, ABTS•+ and •OH, and ferric reducing ability were superior markedly compared to two polysaccharides and ascorbic acid reference. The CPGP-2-I also exhibited most potent inhibitory ability on AGEs formation with IC50 value of 148.12 μg/mL. There was positive correlation between biological activity with mannose and galactose. Our data suggests that the stronger potential of polysaccharides on antioxidant and anti-glycation activities could be due to presence of higher mannose and galactose residues in backbones.