Abstract
Purpose: To isolate polyhydroxyalkanoates (PHA)-producing bacterial strains from contaminated soil using industrial wastewater and glucose as carbon soured by Macrogen sequencing. Two different sources, namely, glucose and wastewater were used to ces.Methods: The strains were isolated and identified as Pseudomonas, Bacillus, Enterobacter, Exiguobacterium and Stenotrophomonas using biochemical tests and further confirmevaluate and compare the use of wastewater as a carbon source for PHA production. The biomass obtained was analyzed by Fourier transform infra-red (FTIR) to identify the presence of PHA in it. Afterwards, PHA extraction was carried out and then gas chromatography (GC) performed to identify PHA monomers.Results: Utilization of glucose resulted in the production of PHB, while wastewater yielded copolymers poly-3 hydroxybutyrate-co-3hydroxyvalerate P(3HB-co-3HV) due to its content of volatile fatty acids such as acetic acid, propionic acid and butyric acid, which led to the production of different types of polymers. The maximum PHA production was 41 ± 0.22 % obtained for Stenotrophomonas (SM03) using 2 % glucose as carbon source while for wastewater, maximum production was achieved by the Pseudomonas strain (SM01).Conclusion: Wastewater is produced in large quantities daily during various activities and therefore can be used as a cheap carbon source for the production of valuable products such as PHA.Keywords: Polyhydroxyalkanoates, Wastewater, Glucose, Pseudomonas strain, Stenotrophomonas
Highlights
Polyhydroxyalkanoates (PHA), produced by around 300 different bacterial strains, serve as carbon and energy storage material and are similar to synthetic plastics in physical and chemical properties
PHA production in industries is carried out using pure microbial cultures, the maintenance of which is the main component of the high cost of PHA production
This contributes to the high cost of pure-culture PHA relative to conventional plastics [2,3]
Summary
Polyhydroxyalkanoates (PHA), produced by around 300 different bacterial strains, serve as carbon and energy storage material and are similar to synthetic plastics in physical and chemical properties. They are considered to be a good alternative to petrochemical plastics [1]. PHA production in industries is carried out using pure microbial cultures, the maintenance of which is the main component of the high cost of PHA production. This contributes to the high cost of pure-culture PHA relative to conventional plastics [2,3]. The use of mixed microbial cultures in sludge or wastewater is one of such approaches as it eliminates the need for media and equipment sterilization [4,5]
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