Abstract
Plum pox virus (PPV) was found naturally infecting blackthorn (Prunus spinosa L.) plants in different regions in Hungary. The virus was identified on the basis of reactions with indicator plants, by DAS-ELISA tests and by RT-PCR. PPV isolated from blackthorn (PPV-BT-H) caused yellow lesions with a necrotic centre on Chenopodium foetidum L. indicating that it belongs to the intermediate pathotype. The coat protein gene of the blackthorn isolate was cloned, sequenced and compared with other PPV sequences. The BT-H isolate and the Hungarian plum isolate SK68 belong to different subclusters of the M group in contrast to the Hungarian almond isolate (PA) which belongs to the D group. Detecting PPV on blackthorn demonstrated that this plant may be an important source and reservoir for PPV in Hungary.
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