Characterization of plastics and their ecotoxicological effects in the Lambro River (N. Italy)

Abstract

This study had the dual objective of both the qualitative and quantitative assessment of plastic mixtures sampled in 5 different sites located along the Lambro River (northern Italy), and the contemporarily determination of the ecotoxicological effects of the same mixtures sampled, through 21-day laboratory exposures of the freshwater bivalve Dreissena polymorpha. The monitoring survey was carried out by a Fourier Transform Infrared Microscope System, while the ecotoxicological assessment was performed by the mussel mortality, a biomarker suite and the proteomics. The main results of the monitoring have highlighted some critical points, related to the concentration of plastics detected at Milan and, especially at the southernmost sampling station, where a daily flow of more than 6 million plastic debris has been estimated, ending directly into the Po River, the main Italian river. The ecotoxicological analysis highlighted how the toxicity is not exclusively due to the plastic concentration, but that the different characteristics of the polymers probably become more important. Furthermore, we observed an extensive mortality of bivalves exposed to the sampled mixtures in the two southernmost sampling stations, while the battery of biomarkers and the results of proteomics have highlighted how the sampled plastic mixtures caused an imbalance in the redox state, already indicated as a classic effect due to plastic exposure, but also an impact on energy stock and on some fundamental cellular pathways always linked to energy metabolism.