Characterization of physiological traits during development of the recalcitrant seeds of Quercus serrata.

Abstract

We investigated how developmental stage affects seed traits, including the relative level of desiccation tolerance of Quercus serrata. We tested the hypothesis that the relative level of desiccation tolerance is a quantitative trait associated with seed development and that a maximum relative level of desiccation tolerance is reached during development. Seed growth and physiological traits of Q. serrata from a subtropical forest were examined in detail during the developmental process. During seed development, the relative level of desiccation tolerance and other seed traits of Q. serrata varied. Dry matter accumulation in seed components increased rapidly beginning in mid-August, and moisture content declined. At the peak period of seed dispersal in late September, seeds were fully mature, with 100% germination. Relative level of desiccation tolerance increased up to the point of peak dispersal; however, at this time seeds were still recalcitrant. Post-mature development was accompanied by further increases in seed dry matter and decreases in moisture content, which led to a decrease in seed germination and relative level of desiccation tolerance. Our results suggest that in species with recalcitrant seeds, the relative level of desiccation tolerance and other seed traits are quantitative at the intraspecific level. The relative level of desiccation tolerance for recalcitrant seeds does not increase infinitely during phase II of development. There is a maximum relative level of desiccation tolerance in recalcitrant seeds within a species.