Characterization of physiological properties of bacteria isolates TM4 and BNt8 in biopesticide formulas

Abstract

Some physiological characteristics can be used to determine the antagonistic ability of a bacterium, was the ability of bacteria to produce extracellular enzymes (chitinase, proteases, and cellulose), hydrogen cyanide (HCN), phosphate solvents, and fluorescence activities. The aim of the study was to examine the physiological characteristics of bacterial isolates TM4 and BNt8 biopesticide formulations. The experiment was conducted at the Plant Pathology Laboratory and greenhouse of Indonesian Cereals Research Institute at Maros South Sulawesi. The results showed that the two isolates tested for physiological characteristics using specific media had chitinolytic, proteolytic, cellulolytic, phosphate and potassium activity. This result was seen from the formation of a clear zone. Based on the results of in vitro tests using a completely randomized design, it was found that the two bacterial isolates tested were able to inhibit the growth of R. solani pathogen with inhibitory levels by TM4 isolates ranging from 13-26% and inhibition of BNt8 isolates around 11-38% respectively. The percentage of bacterial inhibition on PDA media was higher than on media PDA and PDA + TSA. From the field test on maize plants, it was found that the two bacterial isolates tested could significantly increase plant growth.