Abstract
Bio peptone is a combination of enzymatic digest of animal tissues and casein; and generally used for the growth of several varieties of microbes. The aim of present study was to investigate the impact of biofield energy treatment on the physicochemical and spectroscopic properties of bio peptone. The present study was carried out in two groups i.e. control and treated. The control group was kept without treatment, while the treated group was subjected to Mr. Trivedi’s biofield energy treatment. Subsequently, both the samples were assessed using numerous analytical techniques. The X-ray diffractograms (XRD) showed the halo patterns of XRD peaks in both the samples. The particle size analysis exhibited about 4.70% and 17.58% increase in the d50 (average particle size) and d99 (particle size below which 99% particles are present), respectively of treated bio peptone as compared to the control. The surface area analysis revealed the 253.95% increase in the specific surface area of treated sample as compared to the control. The differential scanning calorimetry (DSC) analysis showed the 29.59% increase in the melting temperature of treated bio peptone sample as compared to the control. Thermogravimetric analysis (TGA) showed the increase in onset of degradation temperature by 3.31% in the treated sample with respect to the control. The Fourier transform infrared (FT-IR) study revealed the changes in the wavenumber of functional groups such as O-H stretching from 3066 cm-1 to 3060 cm-1; C-H stretching from 2980, 2893, and 2817 cm-1 to 2970, 2881, and 2835 cm-1, respectively; N-H bending from 1589 cm-1 to 1596 cm-1; C=C stretching from 1533 cm-1 to 1525 cm-1; and P=O stretching from 1070 cm-1 to 1078 cm-1 in treated sample as compared to the control. The UV-vis spectroscopy showed the similar patterns of absorbance maxima (λmax) i.e. at 259 nm and 257 nm in both the control and treated samples, respectively. Overall, the analytical results suggested that Mr. Trivedi’s biofield energy treatment has substantial effect on physicochemical and spectral properties of bio peptone. Owing to this, the treated bio peptone might be more effective as culture medium than the corresponding control.
Highlights
A culture or growth medium is a liquid or gel, which is intended to support the growth of microorganism or cell [1]
The X-ray diffractograms (XRD) study suggested that biofield energy treatment did not induce any changes in the crystal structure of bio peptone with respect to the control sample
The particle size analysis showed the increase in the particle sizes i.e. d50 and d99 of the treated bio peptone as compared to the control
Summary
A culture or growth medium is a liquid or gel, which is intended to support the growth of microorganism or cell [1]. It is processed cautiously to enhance the nutritive values for proper growth requirement of wide variety of microorganisms It offers a wide spectrum of amino acids and peptides and can be used in numerous culture media preparation [5]. The bio peptone meets the nutritional requirements, which are not provided by meat peptone or casein hydrolysate individually It can be used for the culture of fastidious microorganisms. Consciousness is one of the possible mechanisms among the several proposed one to support the biofield energy therapies It includes healer’s intent to heal, and may interact with the physical realm [13]. Based on the above mentioned literature reports on biofield energy treatment and the uses of bio peptone in the culture media, the present study was designed to investigate the effect of biofield energy treatment on bio peptone. The data of treated sample were compared with that of control sample
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