Characterization of phosphorus forms in lake macrophytes and algae by solution (31)P nuclear magnetic resonance spectroscopy.

Abstract

Debris from aquatic macrophytes and algae are important recycling sources of phosphorus (P), which can result in continuing blooms of algae by recycling bioavailable P in the eutrophic lakes. However, knowledge of forms of P in aquatic macrophytes and algae and their contribution to internal loads of P in lakes is limited. Without such knowledge, it is difficult to develop appropriate strategies to remediate and or restore aquatic ecosystems that have become eutrophic. Therefore, in this work, P was extracted from six types of aquatic macrophytes and algae collected from Tai Lake of China and characterized by use of solution (31)P-nuclear magnetic resonance (NMR) spectroscopy. When extracted by 0.5M NaOH-25mM EDTA, extraction recovery of total P(TP) and organic P(Po) exceeded 90%. Concentrations of Po in algae and aquatic macrophytes were 5552mgkg(-1) and 1005mgkg(-1) and accounted for 56.0 and 47.2% of TP, respectively. When Po, including condensed P, was characterized by solution (31)P-NMR Po in algae included orthophosphate monoesters (79.8%), pyrophosphate (18.2%), and orthophosphate diester (2.0%), and Po in aquatic macrophytes included orthophosphate monoesters (90.3%), pyrophosphate (4.2%), and orthophosphate diester (5.5%). Additionally, orthophosphate monoesters in algal debris mainly included β-glycerophosphate (44.1%), α-glycerophosphate (13.5%), and glucose 6-phosphate (13.5%). Orthophosphate monoesters in aquatic macrophytes mainly included β-glycerophosphate (27.9%), α-glycerophosphate (24.6%), and adenosine 5' monophosphate (8.2%). Results derived from this study will be useful in better understanding nutrient cycling, relevant eutrophication processes, and pollution control for freshwater lakes.