Abstract
The rhizosphere soil is a source for a diversity of microorganisms which play a vital role in the enhancement of plant health through the mechanism of symbiotic interaction thereby influencing the plant growth. The present study aimed at isolating potential phosphate solubilizing rhizobacteria from pea (pisum sativa) crop for which, rhizosphere soil samples were collected from two pea growing districts of Kashmir valley viz. Srinagar and Baramulla. Isolates were cultured on nutrient agar medium followed by serial dilutions and different colonies with morphological variations were isolated from each dilution. A total of 80 bacteria were isolated and maintained as pure cultures. Out of the 80 isolates, 25 strains showed phosphate solubilizing ability and amongst them, 9 were highly potential which were subjected to morphological and biochemical characterization. Furthermore, the potential bacteria were analysed for Indole Acetic Acid (IAA) production, which was found to be directly proportional to the plant growth promotion. After screening the best nine isolates PGP1, PGP10, PGP28, PGP40, PGP49, PGP52, PGP60, PGP72 and PGP75 were selected for their effect on growth and yield in field conditions using pea as test crop.
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