Abstract
Phenol is one of the greatest menaces among the industrial pollutants. A treatment method which utilizes agricultural wastes in a simple manner has become the need of the hour. In this investigation, details on the extraction, optimization of parameters and study of detoxification potential of the peroxidase enzyme obtained from baby corn waste have been elaborated. The enzyme has been extracted from both corn silk and husk, and studies have been conducted on both the samples. Estimation of amount of protein and enzyme activity has shown promising results, and the conditions optimized are easily attainable at larger scales, making this work feasible for scale-up.
Highlights
Management and processing of the waste from different industries are the major problem in the different parts of the world (Anupama et al 2013)
The AR-grade chemicals used in this work and supplier details are as follows: ammonium sulphate (Qualigens), calcium chloride (Merck), guaiacol (Srichem), hydrogen peroxide, catechol, phenol (Nice Chemicals), anhydrous sodium carbonate (Rankem), sodium bicarbonate, 4-aminoantipyrine, boric acid, potassium ferricyanide, potassium dihydrogen phosphate (HiMedia Laboratories), di-potassium hydrogen phosphate (S D Fine-Chem Ltd), BSA (Merck), sodium hydroxide (Merck), sodium bicarbonate, copper sulphate, sodium potassium tartrate (Merck) and Folin reagent (Merck)
Considering all the optimized conditions and the extent of detoxification, it can be concluded that the peroxidase enzyme obtained from Zea mays waste can be used as an effective alternative to other methods
Summary
Management and processing of the waste from different industries are the major problem in the different parts of the world (Anupama et al 2013). The harmful impact of molecules that are generated from various different process industries pollutes air and water by its dangerous and mutagenic properties (Busca et al 2008). Major organic pollutants include phenol and its derivatives like chlorophenol, cresols, orthophenols, nitro phenols, etc., produced by various industrial activities, such as coal mining, petroleum refining, resin, plastic and pharmaceutical production, wood preservation, metal coating and textile dyeing (Varsha et al 2011). In addition to toxic effects, phenolic compounds generate an Methods to remove phenols from industrial wastewater are classified as conventional and advanced methods. Enzymes may present balance over traditional technologies and over microbial remediation. All these characteristics turn enzymes into eco-friendly catalysts, and enzymatic
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